Tissue Culture Method for Bovine Mammary Tissue: Lactose Synthesis

Isolation of Adipose Tissue Stem Cells with Organ Culture Method

Background  Isolation of adipose tissue mesenchymal stem cells (MSCs) with current enzymatic methods has some limitations. For example, it is costly and time-consuming and results in a heterogeneous cell population that making compromise proliferation and differentiation of MSCs. Also, it is accompanied with the increased risk of culture contaminations because of several handling steps.In this...

The subcellular distribution of the A and B proteins of lactose synthetase in bovine and rat mammary tissue.

The subcellular distribution of the A and B proteins of lactose synthetase (EC 2.4.1. c) has been determined in bovine and rat mammary tissue. The A protein is mainly associated with the microsomal fraction, whereas the B protein is distributed between the microsomal and soluble fraction. The B protein is more readily dissociated from the microsomes than the A protein. Microsomes present in mil...

Tissue Culture

Dr. Willmer's second edition of this little book fulfills his stated purpose ... to correlate some of the numerous fragments of scattered data which have so far been obtained and to orientate them in such a way as to illustrate the more fundamental contributions which the method has made to physiological knowledge, and to show the manner in which a change of outlook has already been brought abo...

Developmental regulation of mammary-derived growth inhibitor expression in bovine mammary tissue

The cDNA for a previously described growth inhibitor, designated as mammary-derived growth inhibitor (MDGI) (Grosse, R., and P. Langen. 1989. In Handbook of Experimental Pharmacology. In press) has been cloned from a plasmid library which was derived from terminally differentiated bovine mammary gland. Sequencing of the cDNA showed an open reading frame coding for a protein of 133 amino acids. ...

Adaptation of Horsesickness Virus to Tissue Culture